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Purified Rat Anti-Mouse CD4

纯化大鼠抗小鼠CD4

公司名称: BD
产品编号: 553727
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Enrichment of Cells of Interest from Heterogeneous Murine Cells with BioMag Goat Anti-rat IgG
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Date:
2012-04-05
[Abstract]  BioMag Goat Anti-Rat IgG is a standard BioMag particle coated with polyclonal goat anti-rat IgG antibodies and is highly suited for use in cell sorting methods where a rat IgG antibody is used as a primary antibody. BioMag Goat Anti-Rat IgG can be used to separate the cells of interest from a heterogeneous cell population using negative selection. BioMag Goat Anti-Rat IgG can also be used as a secondary antibody in enzyme immunoassays and radioassays that utilize a rat IgG primary monoclonal antibody.
The efficiency of enrichment by negative selection depends on antigen availability and ...
[摘要]  BioMag羊抗鼠免疫球蛋白 是一个标准的多克隆包衣的BioMag颗粒,羊抗大鼠免疫球蛋白抗体。它高频的被用于以大鼠免疫球蛋白为通用抗体的细胞分类。BioMag羊抗大鼠免疫球蛋白可以通过阴性选择用来从异源的细胞群分离目的细胞。BioMag羊抗大鼠免疫球蛋白也可以在酶联免疫中作为二抗,在放射性检测中使用大鼠免疫球蛋白单克隆抗体。
阴性选择的富集效率取决于抗原的有效性和细胞总数。作者对于从仅含大约2%DN细胞的总胸腺细胞中富集小鼠CD4和CD8细胞的双阴性(DN)胸腺细胞很有经验。通常经过一轮富集之后收集到的DN细胞需要多于80%的总胸腺细胞。 然而分离效率却远远低于骨髓细胞。

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