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34-1-2S

34-1-2S

公司名称: ATCC
产品编号: HB-79TM
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Two-event Transfusion-related Acute Lung Injury Mouse Model
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2015-06-20
[Abstract]  Transfusion-related acute lung injury (TRALI) is defined as acute lung injury that occurs within 6 hours of a blood product transfusion. TRALI continues to be a leading cause of transfusion-related mortality and we have developed a mouse model of TRALI to better understand the mechanisms by which injury occurs and to test therapeutic approaches. Our model is a two-event model based on immune priming and the challenge of BALB/c wild-type mice with cognate MHC Class I monoclonal antibody (MHC I mAb). Immune priming with LPS mimics the primed state of recipients (first event) that is important ... [摘要]  输血相关的急性肺损伤(TRALI)定义为在血液制品输血的6小时内发生的急性肺损伤。 TRALI仍然是输血相关死亡率的主要原因,我们已经开发了TRALI的小鼠模型,以更好地了解损伤发生的机制和测试治疗方法。 我们的模型是基于免疫引发的双事件模型和具有同源MHC I类单克隆抗体(MHC I mAb)的BALB/c野生型小鼠的攻击。 用LPS的免疫引发模拟对TRALI的发展重要的受体的引发状态(第一事件)。 供体HLA抗体经常涉及TRALI反应,并且同源MHC I类抗体(第二事件)在引发的动物中产生急性肺损伤。 在这里,我们描述一个详细的协议,在动物中具有高重现性。

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