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Author:
Date:
2015-08-05
[Abstract] Over the last decades numerous regulators of angiogenesis have been identified and characterized. Among the others the vascular endothelial growth factor (VEGFA) appears undoubtedly important in several pathophysiological processes. Moreover, VEGFA represents one of the most attractive targets of anticancer therapy, given its major role in the growth and development of different tumor types. Here we describe a method to detect the copy number variation (CNV) status of the VEGFA gene by fluorescence in situ hybridization (FISH). FISH analysis is a reliable method for investigating ...
[摘要] 在过去的几十年中，已经鉴定和表征了许多血管生成的调节剂。 其中血管内皮生长因子（VEGFA）在几种病理生理过程中无疑是重要的。 此外，VEGFA代表抗癌治疗的最有吸引力的靶标之一，因为其在不同肿瘤类型的生长和发展中的主要作用。 在这里我们描述了通过荧光原位杂交（FISH）检测VEGFA基因的拷贝数变异（CNV）状态的方法。 FISH分析是用于研究VEGFA扩增或增加的基因拷贝数的可靠方法，并且可以代表用于研究VEGFA表达水平的失调的免疫组织化学分析的替代方法。
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