| Extraction and Profiling of Plant Polar Glycerol Lipids
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Author:
Date:
2016-06-20
[Abstract] This protocol describes a method to extract total polar glycerol lipids from plant materials, followed by mass spectrometry profiling. Different glycerol lipid classes can be distinguished by their head-groups, which can be profiled automatically and quantitatively by a triple quadrupole mass spectrometry in multiple reaction monitoring (MRM) mode with an autosampler. Comparing with other established methods, such as thin layer chromatography (TLC) separation followed by Gas spectrometry (GC) analysis, this method requires little effort in sample preparation and separation, while the ...
[摘要] 该方案描述了从植物材料提取总极性甘油脂质的方法，随后是质谱分析。 不同的甘油脂类可以通过其头基来区分，其可以通过使用自动进样器的多重反应监测（MRM）模式中的三重四极杆质谱自动和定量地进行概况分析。 与其他已建立的方法，例如薄层色谱（TLC）分离，然后气体光谱（GC）分析相比，该方法在样品制备和分离中几乎不需要付出努力，而分辨率不限于一般脂质类别，而是侧链水平 。 在Welti等人（2002）中描述并且成功地使用该方法来分析在冰冻胁迫下植物脂质的变化。
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| Physicochemical Quantification of Abscisic Acid Levels in Plant Tissues with an Added Internal Standard by Ultra-Performance Liquid Chromatography
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Author:
Date:
2015-09-20
[Abstract] The phytohormone abscisic acid (ABA) is critical for a range of plant responses to the environment, most importantly in closing the stomata of seed plants during drought (Mittelheuser and Van Steveninck 1969; Brodribb et al. 2014). The high precision quantification of this hormone by physicochemical methods is a relatively simple process, essential for studies that aim to investigate the role or action of this hormone in plants. Outlined here is a method for the extraction, purification and quantification of ABA levels in plant tissues. This method involves methanolic extraction of ...
[摘要] 植物激素脱落酸（ABA）对于一系列植物对环境的反应至关重要，最重要的是在干旱期间闭合种子植物的气孔（Mittelheuser和Van Steveninck 1969; Brodribb等人2014） 。 通过物理化学方法对这种激素的高精度定量是一个相对简单的过程，对于旨在研究该激素在植物中的作用或作用的研究是必要的。 这里概述了用于提取，纯化和定量植物组织中ABA水平的方法。 该方法包括从匀浆组织中甲醇提取ABA。 然后通过简单的以太分区方法进行ABA的纯化。 重点放在确定叶中的ABA水平; 然而该方法适用于所有组织类型，包括植物溶质如木质部汁液，种子（干的和绿色的）和组织的大样品例如根系统。
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