| HBV Infection in Human Hepatocytes and Quantification of Encapsidated HBV DNA
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Author:
Date:
2016-01-20
[Abstract] Human hepatic cancer cell lines such as HepG2, Huh7, and HLE cannot get infected with Hepatitis B virus (HBV) due to lack of an HBV receptor(s). Transfection with HBV genome has so far been referred as a tool to mimic HBV infection. However, since sodium taurocholate cotransporting polypeptide (NTCP) was identified as a functional receptor for HBV (Yan et al., 2012), hepatocyte cell lines that were stably transfected with a plasmid for NTCP expression have been used for HBV infection. This protocol is designed for infection with HBV in human hepatocyte cell line HepG2 expressing NTCP ...
[摘要] 人肝癌细胞系如HepG2，Huh7和HLE由于缺乏HBV受体而不能感染乙型肝炎病毒（HBV）。 HBV基因组的转染迄今为止被称为模拟HBV感染的工具。 然而，由于牛磺胆酸钠共转运多肽（NTCP）被鉴定为HBV的功能性受体（Yan等人，2012），已经使用用用于NTCP表达的质粒稳定转染的肝细胞细胞系 为HBV感染。 该方案设计用于在表达人类肝细胞细胞系HepG2的表达NTCP（HepG2-hNTCP-C4细胞; Iwamoto等人，2014）或原代人肝细胞（PHH）的HBV感染。 在本节中，我们还描述了用于评估HBV感染的方法之一：细胞内衣壳化的HBV DNA的定量。
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| Chick Neural Tube Explant Culture
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Author:
Date:
2015-10-05
[Abstract] The neural tube explant culture technique allows in vitro culturing of small pieces of neural tissue isolated from e.g., chick or mouse embryonic tissue in a matrix of collagen for defined periods of time. This method can be used to study the effects of defined molecules on developmental processes such as neural progenitor proliferation and neuronal differentiation and/or survival. Since the explant material can also be prepared from embryonic tissue electroporated with expression vectors, this technique can be adapted to study gene function in the presence of specific ...
[摘要] 神经管外植体培养技术允许在胶原基质中从例如小鸡或小鼠胚胎组织分离的小片神经组织的体外培养定义的时间段。 该方法可用于研究限定分子对发育过程如神经祖细胞增殖和神经元分化和/或存活的影响。 由于外植体材料也可以从用表达载体电穿孔的胚胎组织制备，该技术可以适于在特定环境信号的存在下研究基因功能。 也可以在解剖步骤期间分离神经管的不同区域，允许单独研究神经管的特定区域。 在这里，我们提出了我们已经在几个研究中使用的神经管外植体培养方法（Dias等人，2014; Lek等人，2010; Vallstedt， et al。，2005）。
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