{{'Search' | translate}}
 

Incubator

培养箱

公司名称: Gemini Bio-Products
产品编号: 4294 F1-1
Bio-protocol()
Company-protocol()
Other protocol()

Chick Neural Tube Explant Culture
Author:
Date:
2015-10-05
[Abstract]  The neural tube explant culture technique allows in vitro culturing of small pieces of neural tissue isolated from e.g., chick or mouse embryonic tissue in a matrix of collagen for defined periods of time. This method can be used to study the effects of defined molecules on developmental processes such as neural progenitor proliferation and neuronal differentiation and/or survival. Since the explant material can also be prepared from embryonic tissue electroporated with expression vectors, this technique can be adapted to study gene function in the presence of specific ... [摘要]  神经管外植体培养技术允许在胶原基质中从例如小鸡或小鼠胚胎组织分离的小片神经组织的体外培养定义的时间段。 该方法可用于研究限定分子对发育过程如神经祖细胞增殖和神经元分化和/或存活的影响。 由于外植体材料也可以从用表达载体电穿孔的胚胎组织制备,该技术可以适于在特定环境信号的存在下研究基因功能。 也可以在解剖步骤期间分离神经管的不同区域,允许单独研究神经管的特定区域。 在这里,我们提出了我们已经在几个研究中使用的神经管外植体培养方法(Dias等人,2014; Lek等人,2010; Vallstedt, et al。,2005)。

产品评论