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Hyaluronidase

牛睾丸透明质酸酶

公司名称: Sigma-Aldrich
产品编号: H3506
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Implantation of Human Peripheral Corneal Spheres into Cadaveric Human Corneal Tissue
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Date:
2017-07-20
[Abstract]  Stem and progenitor cells isolated from human limbal tissue can be cultured in vitro as spheres. These spheres have potential for use as transplantable elements for the repopulation of corneal tissue (Mathan et al., 2016). Herein we describe the detailed protocol for the implantation of human corneal spheres into cadaveric human corneal tissue. This protocol describes the procedure for sphere formation and culture, preparation of tissue for sphere implantation, corneal limbus microsurgery and sphere implantation. [摘要]  可以将从人角膜缘组织分离的茎细胞和祖细胞作为球体体外培养。 这些球体有可能用作角膜组织再造的可移植元件(Mathan等人,2016)。 这里我们描述了将人角膜球植入尸体人角膜组织的详细方案。 该协议描述了球形成和培养的过程,球体植入组织的制备,角膜缘显微外科手术和球体植入。
【背景】以前的研究集中在隔离角膜缘细胞,这些细胞完全是上皮细胞(角膜缘干细胞)或基质(角质细胞祖细胞),以破译他们在角膜内稳态和伤口修复中的各自角色。 该方案旨在通过其在培养物中形成球体的功能能力来分离角膜缘细胞,并且其本质将包括上皮和间质的多种细胞,其有助于角膜缘的形成。 在分离这些球体之后,我们正在调查其在植入后角膜修复中的潜在用途。 在这里,我们描述了一种用于将这些球体植入人角膜组织和下游分析的体外手术方案。

Isolation of Joint-infiltrating Cells
Author:
Date:
2016-09-05
[Abstract]  Infiltration of leukocytes into joints is one of the main features of autoimmune inflammatory arthritis. Here, we describe the protocol for isolation of joint-infiltrating cells in mice. This protocol is useful to analyze cell surface antigens and intracellular cytokines by flow cytometry. [摘要]  白细胞渗入关节是自身免疫性炎症性关节炎的主要特征之一。 在这里,我们描述的协议隔离的关节浸润细胞在小鼠。 该方案可用于通过流式细胞术分析细胞表面抗原和细胞内细胞因子。

Preparation of Single Cell Suspensions from Mouse Aorta
Author:
Date:
2016-06-05
[Abstract]  Atherosclerosis is a chronic inflammatory disease of the arterial wall characterized by lipid deposition, plaque formation, and immune cell infiltration. Innate and adaptive immune cells infiltrate the artery during development of the disease. Moreover, advanced disease leads to formation of artery tertiary lymphoid organs in the adventitia (Grabner et al., 2009; Hu et al., 2015). Various and diverse types of immune cells have been identified in the aorta adventitia vs atherosclerotic plaques (Elewa et al., 2016; Galkina et al., 2006; Lotzer et al., 2010; ... [摘要]  动脉粥样硬化是动脉壁的慢性炎性疾病,其特征在于脂质沉积,斑块形成和免疫细胞浸润。先天性和适应性免疫细胞在疾病发展期间浸润动脉。此外,晚期疾病导致外膜中动脉三级淋巴器官的形成(Grabner等人,2009; Hu等人,2015)。已经在主动脉外膜vs动脉粥样硬化斑块中鉴定了各种不同类型的免疫细胞(Elewa等人,2016; Galkina等人,2006; Lotzer等人, 2010; Mohanta等人,2016; Mohanta等人,2014; Moos等人,2010; Mohanta等人,2010; 2005; Srikakulapu et al。,2016; Zhao et al。,2004)。根据动物的年龄,用于获得单细胞悬浮液的方案和小鼠的饮食条件,存在关于主动脉中免疫细胞的数量和亚型的矛盾报告(Campbell等, 2012; Clement等人,2015; Galkina等人,2006; Kyaw等人,2012)。使用不同的方案,主动脉中免疫细胞的数目差异多达十倍(Butcher等,2012; Galkina等,2006; Gjurich等,2015; Grabner等人,2009; ...

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