| Spared Nerve Injury Model of Neuropathic Pain in Mice
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Author:
Date:
2018-03-20
[Abstract] Experimental models of peripheral nerve injury have been developed to study mechanisms of neuropathic pain in living animals. The spared nerve injury (SNI) model in rodents is a partial denervation model, in which the common peroneal and tibial nerves are injured, producing consistent and reproducible tactile hypersensitivity in the skin territory of the spared, intact sural nerve. SNI-operated mice require less force applied to the affected limb to elicit a withdrawal behavior as compared to sham mice. This effect is observed as early as 2 days after surgery and lasts for at least 1 month. ...
[摘要] 等人，1990; Kim和Chung，1992 ）。部分去神经支配模型使研究人员能够研究不同组的神经元细胞和非神经元细胞的结构和功能变化。研究可以在神经性疼痛的起始，进展（也称为急性）和维持（慢性）阶段以及在沿着疼痛途径的不同解剖部位期间进行，包括远侧与近侧周围神经纤维，背根神经节，脊髓绳索，皮质下和皮层区域。备用神经损伤（SNI）模型包括部分神经损伤，其中腓总神经和胫神经受损，在腓肠神经部位产生一致且可再现的疼痛超敏反应（Decosterd和Woolf，2000; Shields等人， ，2003）。该模型已被证明是有力的，证明了机械敏感性和热响应性的行为测量值的实质和长期变化（Bourquin等人，2006）。这些特征与临床描述的神经性疼痛疾病的主要症状密切相似。
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| Isolation, Culture, and Maintenance of Mouse Intestinal Stem Cells
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Author:
Date:
2016-02-20
[Abstract] In this protocol we describe our modifications to a method to isolate, culture and maintain mouse intestinal stem cells as crypt-villus forming organoids. These cells, isolated either from the small or large intestine, maintain self-renewal and multilineage differentiation potential over time. This provides investigators a tool to culture wild type or transformed intestinal epithelium, and a robust assay for stem cell tissue homeostasis in vitro.
[摘要] 在这个协议，我们描述我们修改分离，培养和维持鼠肠干细胞作为隐窝 - 绒毛形成类器官的方法。 从小肠或大肠分离的这些细胞随时间保持自我更新和多向分化潜能。 这为研究人员提供了培养野生型或转化的肠上皮的工具，以及用于体外干细胞组织稳态的稳健试验。
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