| Whole Mammary Gland Transplantation in Mice Protocol
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Author:
Date:
2017-06-05
[Abstract] Whole Mammary Gland Transplantation involves transplanting an excised mammary gland into another, more suitable host. This method can be used to extend the life of a mammary gland past the mouse’s life span by transplanting the mammary gland of an older mouse into a young healthy mouse. As you can see in the video below (Video 1), by attaching it to the abdomen of the mouse, the gland will receive a steady blood supply and both epithelial and stromal cells will remain viable for up to one year. Although this method is not used often, it has been part of several experiments including ...
[摘要] 全乳腺移植涉及将切除的乳腺移植到另一个更合适的宿主中。该方法可用于通过将老鼠的乳腺移植到年轻的健康小鼠中来延长乳腺的寿命，超过老鼠的寿命。在下面的视频（视频1）中可以看到，通过将其附加到鼠标的腹部，腺体将获得稳定的血液供应，并且上皮细胞和基质细胞将保持活力长达一年。虽然这种方法不经常使用，但它已经是几个实验的一部分，包括确定基质或上皮是化学诱导的小鼠乳腺肿瘤发生的主要靶标（Medina和Kittrell，2005）。为了监测移植物，每周触诊肿瘤形成。移植的乳腺也可以每8-10周连续传播一次。将移植腺保持在同一只老鼠不超过一年。
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| Mouse Mammary Intraductal (MIND) Method for Transplantation of Patient Derived Primary DCIS Cells and Cell Lines
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Author:
Date:
2016-03-05
[Abstract] The MIND method involves intraductal injection of patient derived ductal carcinoma in situ (DCIS) cells and DCIS cell lines (MCF10DCIS.COM and SUM225) inside the mouse mammary ducts [Video 1 and Figure 1 in Behbod et al. (2009)]. This method mimics the normal environment of DCIS and facilitates study of the natural progression of human DCIS, i.e., their initial growth as carcinoma in situ within the ducts, followed by invasion into the stroma through the myoepithelial cell layer and basement membrane (Behbod et al., 2009; Valdez et al., 2011). In ...
[摘要] MIND方法涉及管内注射患者来源的导管原位（DCIS）细胞和DCIS细胞系（MCF10DCIS.COM和SUM225）（在小鼠乳腺导管内）[Video 1和FIG 1 in Behbod et al。 >（2009）]。该方法模拟DCIS的正常环境，并且有助于研究人DCIS的自然进展，即它们在导管内原位癌的初始生长，随后是侵入通过肌上皮细胞层和基底膜的基质（Behbod等人，2009; Valdez等人，2011）。为了证明移植手术是成功的，可以早在导管内注射细胞后两周，随后使用人特异性细胞角蛋白5和/或19的苏木精和曙红（H&E）染色和/或免疫荧光染色，切除移植的乳腺[请参见Behbod等人（2009）中的图2-4]。此外，在管内注射后立即在小鼠乳腺导管内存在台盼蓝是注射成功的最佳指示（视频1从4:33秒开始）。
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