| Semi-thin Sectioning, Light and Fluorescence Microscopy of Floral Bud to Study Microspore Development in Arabidopsis
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Author:
Date:
2016-03-05
[Abstract] Pollen grains are male gametophytes produced within the pollen sacs of the anthers of the flower. Recent genetic studies have revealed several components involved in microspore development (Borg et al., 2009; Berger and Twell, 2011), and yet many components controlling microspore development remain to be identified. Semi-thin sectioning of anthers and light and fluorescence microscopy of floral bud (Kim et al., 2015) are the initial key experiments to characterize Arabidopsis mutants and transgenic plants for understanding the roles of new genetic components during ...
[摘要] 花粉粒是在花的花药的花粉囊内产生的雄性配子体。 最近的遗传研究揭示了涉及小孢子发育的几种组分（Borg等人，2009; Berger和Twell，2011），然而仍有许多控制小孢子发育的组分被鉴定。 花药的半薄切片和花芽的光和荧光显微镜检查（Kim等人，2015）是表征拟南芥突变体和转基因植物的初始关键实验 在小孢子发育过程中新遗传组分的作用。 在这里，我们描述了拟南芥花药半花瓣和光和荧光显微镜的半薄切片的协议。
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| Infection Experiments (Hepatitis C Virus)
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Author:
Date:
2015-02-05
[Abstract] The establishment of a cell culture system for hepatitis C virus based on the JFH-1 strain and human hepatoma cell lines has been instrumental for the study of the viral replication cycle. The robustness of the JFH1-based cell culture models enabled many laboratories around the world to perform HCV infections in cell culture, accelerating the identification of cellular and viral targets to develop novel antiviral compounds. Although other robust infection systems based on different molecular clones and different cell lines have been developed since then, here we describe the protocols ...
[摘要] 基于JFH-1毒株和人肝癌细胞系的丙型肝炎病毒的细胞培养系统的建立已经有助于研究病毒复制周期。 基于JFH1的细胞培养模型的鲁棒性使世界上许多实验室在细胞培养物中执行HCV感染，加速细胞和病毒靶标的鉴定以开发新的抗病毒化合物。 虽然基于不同的分子克隆和不同的细胞系的其他强大的感染系统已经发展到今天，在这里我们描述了对应的感染JFH-1和JFH1衍生的病毒在我们的实验室生产病毒库存和持续感染细胞 文化。 我们还描述了用于确定病毒传播能力（多周期感染）以及解剖早期和晚期HCV感染（单周期感染）的实验设置。
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