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pLenti CMV Puro LUC (w168-1)

pLenti CMV Puro LUC(w168-1)

公司名称: Addgene
产品编号: 17477
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In vivo Bioluminescence Imaging of Luciferase-labeled Cancer Cells
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2016-03-20
[Abstract]  Over the past decade, in vivo bioluminescent imaging has emerged as a non-invasive and sensitive tool for studying ongoing biological processes within living organisms (Contag et al., 1997; Contag et al., 1998). Based on the detection and quantitation of the photons produced by the oxidation of luciferin by luciferase enzymes (Harvey, 1927), this technique has proved to be particularly useful in analyzing cancerous cells and monitoring tumor growth (Edinger et al., 1999; Sweeney et al., 1999; Vidal et al., 2015), providing a cost-effective ... [摘要]  在过去十年中,体内生物发光成像已经成为用于研究活生物体内正在进行的生物过程的非侵入性和敏感性工具(Contag等人,1997; Contag et al。,1998)。 基于通过荧光素酶氧化荧光素产生的光子的检测和定量(Harvey,1927),该技术已经证明在分析癌细胞和监测肿瘤生长中特别有用(Edinger等, ,1999; Sweeney等人,1999; Vidal等人,2015),提供了关于疾病在体内如何进展的成本效益的洞察 ,无需连续牺牲动物。 该协议详细描述了在免疫受损的小鼠中获得荧光素酶标记的肿瘤的程序,其可以通过使用IVIS光谱成像仪通过生物发光成像进行研究。

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