Antifungal and Zearalenone Inhibitory Activity of Ocimum sanctum L. Essential Oil on Fusarium graminearum Determined by UHPLC and RT-qPCR
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Author:
Date:
2016-08-05
[Abstract] Fusarium graminearum has been given special attention in the context of agricultural commodities due to its ability to grow in diverse climatic conditions, and to produce different mycotoxins including zearalenone (ZEA) and type-B trichothecenes, which cause ill health effects on humans, animals and plants. The application of synthetic antifungal agents for the control of F. graminearum result in negative health impacts in livestock and humans and the upsurge of resistant organisms as well. Therefore, there is a need to propose proper food grain management practices, ...
[摘要] 由于其在多种气候条件下生长的能力,并且产生不同的真菌毒素,包括玉米赤霉烯酮(ZEA)和B型单端孢霉烯,因此在农业商品的背景下已经特别关注禾谷镰刀菌对人类,动物和植物的健康影响。合成抗真菌剂用于控制F的应用。禾谷镰菌对家畜和人类的健康造成负面影响,以及抗性生物体的高涨。因此,需要提出适当的食物谷物管理实践,包括应用草本抗真菌剂和真菌毒素控制剂以减少产毒的F的生长。禾谷镰菌以及在农产品中生产ZEA。 罗勒属圣地也被称为圣罗勒或图尔西被广泛用作阿育吠陀的药用植物。目前的协议表明量化em的抗真菌活性。圣地牙本香精油(OSEO),如由降低的F所反映的。禾谷镰刀菌生长和ZEA生产。 OSEO的抗真菌活性通过微孔稀释法进行,并通过扫描电子显微镜方法定量进一步验证。通过定量逆转录PCR(RT-qPCR)和超高效液相色谱(UHPLC)方法从F的肉汤培养物分析OSEO对ZEA产生的影响。禾本科。 OSEO的抗真菌毒性功效直接在F上评估。禾谷镰菌接种的玉米谷物。该方案有效地评估OSEO作为抗真菌侵袭和ZE生产的草药拮抗剂的活性。禾本科。该方案可用于测试多种草药化合物对抗F的抗真菌活性。禾谷镰刀菌或对其他霉菌毒素真菌进行修饰,这是对食品安全和加工工业的重要干预,其中真菌侵染是主要关注的问题。
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Extraction and Measurement of Strigolactones in Sorghum Roots
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Author:
Date:
2016-03-20
[Abstract] Strigolactones (SLs) are carotenoid-derived signaling chemicals containing two lactone moieties in their structures and induce seed germination of root parasitic plants, Striga and Orobanche spp. In the rhizosphere, SLs are essential host recognition signals not only for root parasitic plants but also for arbuscular mycorrhizal fungi. In plants, SLs play important roles as plant hormones regulating shoot and root architecture. Plants produce only trace amounts of chemically unstable SLs, which makes it difficult to determine SL contents in plant tissues. Here, we describe ...
[摘要] 独脚金内酯(SL)是类胡萝卜素衍生的信号化学品,其在其结构中含有两个内酯部分,并诱导根寄生植物,Striga和Orobanche的种子发芽。 在根际中,SLs是必不可少的宿主识别信号,不仅对于根部寄生植物,而且对于丛枝菌根真菌。 在植物中,SLs作为调节枝条和根构造的植物激素发挥重要作用。 植物仅产生痕量的化学不稳定的SL,这使得难以确定植物组织中的SL含量。 在这里,我们描述如何提取和量化sorgomol和5-deoxystrigol,主要SLs高粱根中产生。
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