|
Author:
Date:
2017-01-05
[Abstract] Activation of the aryl hydrocarbon receptor (AHR) by endogenous ligands has been implicated in a variety of physiological processes such as cell cycle regulation, cell differentiation and immune responses. It is reported that tryptophan metabolites, such as kynurenine (Kyn) and 6-formylindolo(3,2-b)carbazole (FICZ), are endogenous ligands for AHR (Stockinger et al., 2014). This protocol is designed for treatment with Kyn or FICZ in mouse embryonic fibroblasts (MEFs) or primary peripheral monocytes.
[摘要] 通过内源性配体活化芳基烃受体（AHR）已涉及多种生理过程，如细胞周期调控，细胞分化和免疫应答。据报道，色氨酸代谢物，如犬尿胆碱（Kyn）和6-甲基吲哚（3,2-b）咔唑（FICZ）是AHR的内源性配体（Stockinger等人，2014）。该方案设计用于在小鼠胚胎成纤维细胞（MEF）或初级周边单核细胞中用Kyn或FICZ进行治疗。
背景 色氨酸代谢物如Kyn和FICZ是生理条件下AHR的内源性配体。 Kyn由色氨酸-2,3-双加氧酶（TDO）和/或吲哚胺-2,3-双加氧酶1和2（IDO1 / 2）产生，并有助于抑制抗肿瘤反应和恶性进展（Stockinger等人，2014）。 ...
|