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Methanol

甲醇

公司名称: Thermo Fisher Scientific
产品编号: A452
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Preparation of Respiratory Syncytial Virus with High or Low Content of Defective Viral Particles and Their Purification from Viral Stocks
Author:
Date:
2016-05-20
[Abstract]  Respiratory syncytial virus (RSV) belongs to the paramyxovirus family that includes many clinically relevant viruses, such as the human metapneumovirus and measles. RSV infection can cause severe disease in infants, the elderly, and some immunocompromised adults. During RSV replication, a series of truncated forms of the viral genome is generated. These truncated viral genomes are known as defective viral genomes (DVGs) and are generated by many viruses (Lazzarini et al., 1981; Rao and Huang, 1982; Prince et al., 1996; Sun et al., 2015; Tapia et al., ... [摘要]  呼吸道合胞病毒(RSV)属于包括许多临床相关病毒(例如人偏肺病毒和麻疹)的副粘病毒家族。 RSV感染可以在婴儿,老年人和一些免疫受损的成人中引起严重的疾病。在RSV复制期间,产生一系列截短形式的病毒基因组。这些截短的病毒基因组被称为缺陷型病毒基因组(DVG),并且由许多病毒产生(Lazzarini等人,1981; Rao和Huang,1982; Prince等人, ,1996; Sun等人,2015; Tapia等人,2013)。 DVG可以限制全长病毒的复制,并且是针对RSV的先天免疫应答的主要天然触发因子(Sun等人,2015; Tapia等人 ,2013)。在这里,我们详细讨论如何准备具有高或低含量的DVG的RSV股票,以及如何从富含缺陷病毒颗粒的RSV原液中纯化含有DVG的缺陷病毒颗粒。这些程序可用于制备实验室研究所需的病毒原液和有缺陷的病毒颗粒。简言之,在HEp2细胞中产生不同的RSV种群,其通常用于在实验室中扩增该病毒。为了产生具有高含量DVG的RSV原液,HEp2细胞用高多重感染(MOI)多次感染,然后使用梯度离心纯化含有DVG的病毒颗粒。这里描述的方法具有四个部分:1.具有低DVG含量(RSV-LD)的种子RSV原种的扩增,2.具有高DVG含量(RSV-HD)的原种的产生,3.通过梯度离心,4.纯化DVG的表征。

Extraction and Quantification of Tryptophan and Kynurenine from Cultured Cells and Media Using a High Performance Liquid Chromatography (HPLC) System Equipped with an Ultra-Sensitive Diode Array Detector
Author:
Date:
2016-04-05
[Abstract]  Evidence of the involvement of tryptophan and its metabolite, kynurenine, in various biological processes including cancer is constantly expanding. Analysis of cell extracts and culture media can allow for quick snapshots of the metabolic fluctuations occurring in vitro. Here, we describe a method for metabolite extraction from mammalian cells and analysis of extracted metabolites and cell culture media by HPLC with detection using an ultra-sensitive diode array detector. [摘要]  色氨酸及其代谢物,犬尿氨酸参与包括癌症在内的各种生物过程的证据不断扩大。 细胞提取物和培养基的分析可以允许在体外发生的代谢波动的快速快照。 在这里,我们描述了一种从哺乳动物细胞代谢物提取的方法,提取代谢物和细胞培养基通过HPLC检测使用超敏二极管阵列检测器分析。

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