{{'Search' | translate}}
 

Mitomycin C

丝裂霉素C.

公司名称: AppliChem
产品编号: A2190
Bio-protocol()
Company-protocol()
Other protocol()

In vitro Tumor Cell Migration Assay Using ThinCertsTM (Transwells)
Author:
Date:
2016-06-05
[Abstract]  The high migration rate of tumor cells often results in poor prognosis for the survival of the patients. Here, we describe a protocol to measure the migration of cells using a quantitative assay. The relative tumor cell migration was measured using ThinCertsTM cell culture inserts and a lactate dehydrogenase (LDH) assay to quantify the relative cell number. The quantification of the migration with the LDH kit is much more precise than other methods using i.e. crystal blue to count the cells. [摘要]  肿瘤细胞的高迁移率通常导致患者的生存的不良预后。 在这里,我们描述了使用定量测定测量细胞迁移的协议。 使用ThinCerts TM细胞培养插入物和乳酸脱氢酶(LDH)测定来测量相对肿瘤细胞迁移以定量相对细胞数。 使用LDH试剂盒的迁移的定量比使用结晶蓝计数细胞的其它方法精确得多。

产品评论