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Home-made Microinjection needles from Borosilicate Glass with Filament, OD 1 mm, ID 0.5 mm, length 10 cm, Fire polished

玻璃毛细管

公司名称: Sutter Instrument
产品编号: BF100-50-10
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In utero Electroporation of Mouse Cerebellar Purkinje Cells
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2016-06-05
[Abstract]  In utero electroporation (IUE) of mouse cerebellar Purkinje cells allows high expression levels of transgenes without toxicity (Nishiyama et al., 2012). This technique is suitable for co-transfection of multiple plasmid genes. Therefore, it is useful to express various sets of genes such as drug-inducible Cre/loxP constructs and CRISPR/Cas9 genome editing constructs (Takeo et al., 2015). Murine Purkinje cells arise from subventricular zone of fourth ventricle at embryonic day (E) 10-12. IUE at E11.5 into fourth ventricle results the most efficient transfection into ... [摘要]  在小鼠小脑浦肯野细胞的子宫电穿孔(IUE)中,允许没有毒性的转基因的高表达水平(Nishiyama等,2012)。 该技术适用于多重质粒基因的共转染。 因此,表达各种基因如药物诱导型Cre / loxP构建体和CRISPR / Cas9基因组编辑构建体是有用的(Takeo等,2015)。 小鼠浦肯野细胞起因于胚胎期(E)10-12的第四脑室下室。 IUE在E11.5进入第四脑室导致最有效的转染到浦肯野细胞。

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