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Author:
Date:
2012-05-20
[Abstract] Liver is the major site for glycogen storage. Glycogen content can be significantly altered upon disruption of glucose homeostasis in metabolic syndromes, such as diabetes. Glycogen content can be determined by an acid-hydrolysis method (Passonneau and Lauderdale, 1974). Basically, glucose, the hydrolysis product of glycogen, is converted into glucose-6-phosphate (G-6-P) by hexokinase in the presence of ATP. With the supply of NADP, G-6-P is further converted into 6-phosphogluconic acid by G-6-P dehydrogenase (G-6-PDH), while production of NADPH can be measured spectrophotometrically. Our ...
[摘要] 肝脏是主要的糖原储存地方。在代谢综合征中例如糖尿病，通过破坏葡萄糖的平衡可以显著改变糖原的含量。糖原含量可以用酸水解的方法测定 [1].通常，糖原水解后的产物葡萄糖在有ATP存在下在己糖激酶的作用下转化为6-磷酸葡萄糖。在NADP参与下,G-6-P在 G-6-P脱氢酶的作用下进一步转化为6-磷酸葡萄糖酸，同时NADPH可以通过分光光度计检测到。我们实验室利用这种方法证明在糖尿病Perk灭火鼠中肝糖原显著的上升[2]。
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