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Crile-Wood Needle Holders

丁腈针架

公司名称: Fine Science Tools
产品编号: 12003-15
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In utero Electroporation of Mouse Embryo Brains
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2012-07-20
[Abstract]  This is a non-invasive technique to introduce transgenes into developing brains. In this technique, DNA is injected into the lateral ventricle of the embryonic brains, and is incorporated into the cells through electroporation. Embryos then continue their development in normal conditions in vivo. The effects of genes of interest can be evaluated at certain time points after in utero electroporation. This technique allows acute knockdown or over expression of genes of interest. Compensatory effects from other genes are less likely to happen; it also circumvents possible ... [摘要]  这是一种无侵入性的将基因转入发育的大脑的转基因技术。在这个方法中,DNA被注射入胚胎脑的侧脑室,通过电穿孔的方法被吸收到细胞中。然后,胚胎细胞在体内正常条件继续发育。可以在固定的时间点检测转入的目的基因的在子宫电穿孔中的影响。这种基因允许精确的下调或过表达目的基因。来自于其他基因的补偿性作用很少发生;同时也能避免可能的慢性的有害影响。

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