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Author:
Date:
2012-05-20
[Abstract] Clonogenic assays serve as a useful tool to test whether a given cancer therapy can reduce the clonogenic survival of tumor cells. A colony is defined as a cluster of at least 50 cells that can often only be determined microscopically. A clonogenic assay is the method of choice to determine cell reproductive death after treatment with ionizing radiation, but can also be used to determine the effectiveness of other cytotoxic agents. The following protocol has been modified from a published version (Franken et al., 2006).
[摘要] 克隆生成测定作为一个有用的工具用于检测在肿瘤治疗后是否减少了肿瘤细胞克隆存活率，一个群体并定义为由至少50个细胞形成的簇，这个可以在显微镜下测定 。克隆生成测定是一种测定细胞在电离辐射处理下的生殖死亡方法，也可以用于测定其它的细胞毒剂的效应。, 下面的方法是发表版本的改进版(Franken, Rodermond et al. 2006).
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