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SuperScript® II Reverse Transcriptase

SuperScript ® II Reverse Transcriptase

公司名称: Thermo Fisher Scientific
产品编号: 18064022
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Preparation of cDNA Library for dRNA-seq
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Date:
2012-12-05
[Abstract]  microRNAs (miRNAs) are ubiquitous regulators of gene expression in eukaryotic organisms, which guide Argonaute proteins (AGO) to cleave target mRNA or inhibit its translation based on sequence complementarity. In plants, miRNA directed cleavage occurs on the target mRNA at about 10 to 11 nucleotide (nt) up stream to the site where the 5’ end of miRNA binds. Sequencing of the miRNA directed cleavage products (degradome) is widely employed as a way to both verify bioinformatic predictions of miRNA mediated regulation and identify novel targets of known miRNAs. Here we describe a protocol for ... [摘要]  该实验方案的中文版正在准备中...

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