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BioScriptTM

BioScript TM

公司名称: Bioline
产品编号: BIO-27036
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Cross-linked RNA Immunoprecipitation
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Date:
2013-03-05
[Abstract]  This method is for the immunoprecipitation of Flag-Tagged RNA binding proteins from mammalian cell lines and isolation of the bound RNAs for analysis by quantitative real-time PCR. The RNA binding protein of interest should be tagged with the M2 Flag-tag and expressed in the mammalian cell line of interest (Knuckles et al., 2012). However, specific antibodies for the protein of interest can be used in conjunction with Sepharose G-beads. [摘要]  该方法用于来自哺乳动物细胞系的Flag-标记的RNA结合蛋白的免疫沉淀和分离结合的RNA,用于通过定量实时PCR分析。 感兴趣的RNA结合蛋白应该用M2 Flag标签标记并在感兴趣的哺乳动物细胞系中表达(Knuckles等人,2012)。 然而,感兴趣的蛋白质的特异性抗体可以与Sepharose G-珠结合使用。

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