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Drosophila-SFM (1X)

果蝇-SFM(1x)

公司名称: Thermo Fisher Scientific
产品编号: 10797025
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Transfection of S2 Cell with DNA Using CellFectin Reagent
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Date:
2012-03-05
[Abstract]  This method provides a step-by-step guide to transfecting Drosophila S2 cells with the pRmHA-3 (or similar) vector with insert of choice (in this case SDF-1β-FLAG) and generating a stable cell line. This cell line is then capable of producing the protein of interest under inducible conditions by addition of copper sulfate, which can then be purified and used as desired. This protocol provides an example to finding out when your peak protein production occurs and a method for determining optimal selection conditions. [摘要]  这个方法提供一步一步的指导用选择插入(在这里是SDF-1β-FLAG) pRmHA-3 (or similar)载体转染果蝇S2细胞,生成一个稳定的细胞系。这个细胞系有能力在添加硫酸铜及合适的诱导条件下产生蛋白质,蛋白可以被纯化及根据需要使用。这个方法提供一个找到当峰蛋白产出发生的例子及确定合适的选择条件。

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