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Author:
Date:
2013-06-05
[Abstract] Transposable elements (TEs) are a major component of all genomes, thus the epigenetic mechanisms controlling their activity is an important field of study. Cytosine methylation is one of the factors regulating the transcription and transposition of TEs, alongside Histone modifications and small RNAs. Adapter PCR-based methods [such as Amplified Fragment Length Polymorphism (AFLP)] have been successfully used as high-throughput methods to genotype un-sequenced genomes. Here we use methylation-sensitive restriction enzymes, in combination with PCR on adaptor-ligated restriction fragments, to ...
[摘要] 转座元件（TE）是所有基因组的主要组成部分，因此控制其活性的表观遗传机制是重要的研究领域。 胞嘧啶甲基化是调节TE的转录和转座，与组蛋白修饰和小RNA一起的因素之一。 基于适配子PCR的方法[例如扩增片段长度多态性（AFLP）]已经成功地用作高通量方法来对未测序的基因组进行基因分型。 在这里我们使用甲基化敏感的限制酶，结合适配器连接的限制性片段上的PCR，以评估基因组DNA样品之间的TE的表观遗传变化。
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