|
Author:
Date:
2013-07-20
[Abstract] DNase I footprinting is used to precisely localise the position that a DNA binding protein, e.g. a transcription factor, binds to a DNA fragment. A DNA fragment of a few hundred bp is labelled at one end and then incubated with the proteins suspected to bind. After a limited digestion with DNase I, the reaction is quenched, DNA is precipitated and analysed on a denaturing polyacrylamide gel. This protocol uses 32P-radioactively labeled DNA.
[摘要] DNase I足迹用于精确定位DNA结合蛋白例如转录因子结合DNA片段的位置。 在一端标记几百bp的DNA片段，然后与怀疑结合的蛋白质一起孵育。 在用DNA酶I有限消化后，淬灭反应，沉淀DNA并在变性聚丙烯酰胺凝胶上分析。 该方案使用32-放射性标记的DNA。
|