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PEN foil 2.0 UM covered slides

PEN箔2.0 UM覆盖载玻片

公司名称: ibidi
产品编号: 11505158
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Total RNA Isolation after Laser-capture Microdissection of Human Cervical Squamous Epithelial Cells from Fresh Frozen Tissue
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Date:
2013-08-05
[Abstract]  As most tissue specimens contain a mixture of different cell types including epithelial cells, stromal cells and immune cells, selection of the cells of interest is of utmost importance for the accurate determination of gene/microRNA expression. Laser capture microdissection enables the researcher to obtain homogeneous ultrapure cell selections from heterogeneous starting material. The following protocol was optimized for the isolation of total RNA from cervical (premalignant) squamous epithelial cells from fresh frozen biopsy specimens. [摘要]  由于大多数组织标本含有不同细胞类型的混合物,包括上皮细胞,基质细胞和免疫细胞,所以选择感兴趣的细胞对于精确测定基因/微小RNA表达是至关重要的。 激光捕获显微切割使研究者能够从异质起始材料中获得均匀的超纯细胞选择。 以下方案针对从新鲜冷冻的活检标本的宫颈(恶化前)鳞状上皮细胞中分离总RNA进行了优化。

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