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Anti-Mouse IgG (whole molecule)–FITC antibody produced in goat

在山羊中产生的抗小鼠IgG(全分子)-FITC抗体

公司名称: Sigma-Aldrich
产品编号: F0257
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Cell Proliferation Assay by Flow Cytometry (BrdU and PI Staining)
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2012-04-05
[Abstract]  Cell Proliferation assays include an important set of fluorescence-based tests that can monitor cell health and cell division by evaluating DNA synthesis through thymidine incorporation. Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues. BrdU can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to ... [摘要]  细胞增殖测试是一个重要的基于荧光检测的实验,可以监测细胞健康和通过胸苷掺合来估量DNA合成从而监测细胞分裂。溴脱氧尿苷(5-溴代-2'-脱氧尿苷, BrdU) 是一种合成的核苷酸即胸苷的类似物. BrdU通常被用于检测活组织中再生的细胞。 BrdU可以掺合到复制细胞中新的合成的DNA中去(在细胞周期的S期),在DNA复制是代替胸苷。. BrdU 特异性抗体可以用于检测这个参入的化学品,从而检测出细胞是否在复制其DNA。对抗体的结合需要DNA变形,通常将细胞置于酸性环境或者加热。掺合的BrdU通常通过标记抗BrdU的抗体和DNA染料碘化丙啶(PI)而用流式细胞仪检测来实现对细胞周期的检测。

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