Polypropylene Mesh (105 μm & 500 μm sizes)

聚丙烯网（105μm和500μm尺寸）

公司名称: Thermo Fisher Scientific

产品编号: 146436

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A Fast and Reliable Method to Generate Pure, Single Cell-derived Clones of Mammalian Cells

Zhe Han, Bindhu K. Madhavan, Serap Kaymak, Peter Nawroth and Varun Kumar

Published online: 2022-08-20

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Pancreatic Acinar Cell 3-Dimensional Culture

Author:

Chunjing Qu and Stephen F. Konieczny,

Date:

2013-10-05

[Abstract] Normal pancreatic acinar cells are difficult to maintain on traditional plastic culture surfaces due to their physical properties of housing large quantities of digestive enzymes and the formation of intercellular tight junctions and gap junctions (Apte and Wilson 2005; Rukstalis et al., 2003). However, placing primary acinar cells within a 3-dimensional matrix (3D-culture) maintains the cells for sufficient time so that they can be monitored for physiological changes to different stimuli. We have used a modified collagen 3D-culture system that has been adapted from Means et al. ... [摘要] 正常的胰腺腺泡细胞难以保持在传统的塑料培养表面上，因为其具有容纳大量消化酶和形成细胞间紧密连接和间隙连接的物理性质（Apte和Wilson 2005; Rukstalis等人，，2003）。然而，将原代腺泡细胞放置在三维基质（3D培养）中保持细胞足够的时间，使得它们可以被监测对不同刺激的生理变化。我们已经使用改良的胶原三维培养系统，其已经从Means等人（2005）改编以模拟与胰腺癌发展相关的非常早期的事件。在该模型中，表达Kras ^G12D的表达胰腺腺泡细胞或用EGFR依赖性生长因子（即，TGFα）处理的野生型腺泡细胞转化成导管囊肿，在胰腺上皮内瘤形成（PanIN）和胰腺导管腺癌（PDAC）形成之前的腺泡至导管化生（ADM）阶段（Means等人，2005; Shi等人，/em>，2013）。

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Polypropylene Mesh (105 μm & 500 μm sizes)

聚丙烯网（105μm和500μm尺寸）

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