| Xenograft Tumor Growth Assay
|
|
Author:
Date:
2013-10-20
[Abstract] Chronic inflammation drives initiation of hepatocellular carcinoma (HCC), but the underlying mechanisms linking inflammation and tumor formation remain obscure. In this study, Xenograft tumor assay was used to determine the tumorigenic activity of hepatoma cells with ISX over expression on nude mice in vivo.
[摘要] 慢性炎症驱动肝细胞癌（HCC）的引发，但是连接炎症和肿瘤形成的基础机制仍然是模糊的。 在该研究中，使用软琼脂贴壁非依赖性测定来测定具有ISX的肝癌细胞的肿瘤转化活性超过表达或体外敲低。...
|
|
|
| Western Blot for Detecting Phosphorylated STAT3
|
|
Author:
Date:
2011-08-20
[Abstract] The STAT3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors and is constitutively activated in a number of human tumors and possesses oncogenic potential and anti-apoptotic activities. STAT3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding. Western blot is most commonly used to detect the activation of STAT3 by using an antibody that is specific for the phosphorylated tyrosine705.
[摘要] Stat3转录因子是许多细胞因子和生长因子受体中重要的信号分子，它在许多人类的肿瘤细胞中持续激活，具备潜在的致癌能力和抗凋亡能力。Stat3通过705位酪氨酸磷酸化被激活，进而诱导它的二聚化，核定位以及DNA结合功能。STAT3的活性经常通过能识别705位酪氨酸磷酸化的抗体来检测。
|
|
|