{{'Search' | translate}}
 

Corning® Transwell® polycarbonate membrane cell culture inserts

Corning ® Transwell ®聚碳酸酯膜细胞培养插入物

公司名称: Sigma-Aldrich
产品编号: CLS3421
Bio-protocol()
Company-protocol()
Other protocol()

Transwell Cell Migration Assay Using Human Breast Epithelial Cancer Cell
Author:
Date:
2012-02-20
[Abstract]  Transwell migration assays have been widely used for studying the motility of different types of cells including metastatic cancer cells. The assay is also useful in screens for compounds that act as chemoattractants or inhibitors of chemotaxis for cells. The assay employs a permeable layer of support, usually a tissue-culture-treated microporous membrane, which is positioned between two compartments that mimic two different sets of microenvironments for cell survival/growth. Cells on one side of the membrane, when sensing chemoattractants placed on the other side of the compartment that ... [摘要]  小室迁移实验已被广泛用于研究不同类型包括转移性肿瘤细胞的细胞活力。该实验对检验趋化因子或细胞趋化抑制剂的化合物是有用的。检测依赖于透水层的支持,通常这是组织培养处理的微孔滤膜,它位于两个小室之间,模仿两个不同的微环境对细胞生存/生长。细胞在膜的一侧,从小室的另一侧感应的趋化因子,可以通过微孔膜迁移。被移植的细胞可以用简单的固定/染色的计数方法量化。人类乳腺上皮腺癌MD - 231细胞生长比较快,是转移性的。 以下就以MB - 231细胞细胞株为例描述细胞迁移实验的基本程序。

产品评论