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Author:
Date:
2013-12-05
[Abstract] Outer membrane vesicles (OMVs) are spherical bilayered phospholipids of 20-200 nm in size produced from all Gram-negative bacteria and Gram-positive bacteria investigated to date. Previous biochemical and proteomic studies have revealed that the Gram-negative bacteria-derived OMVs are composed of various components like outer membrane proteins, lipopolysaccharides, outer membrane lipids, periplasmic proteins, DNA, and RNA. Here, in this protocol, we describe the method to isolate the OMVs from the culture supernatant of Escherichia coli (E. coli).
[摘要] 外膜囊泡（OMV）是从所有革兰氏阴性细菌和革兰氏阳性细菌产生的20-200nm的球形双层磷脂，迄今为止研究。 以前的生物化学和蛋白质组学研究已经显示，革兰氏阴性细菌衍生的OMV由各种组分组成，如外膜蛋白，脂多糖，外膜脂质，周质蛋白，DNA和RNA。 在这里，在该方案中，我们描述了从大肠杆菌的培养物上清液中分离OMV的方法（大肠杆菌）。
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