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Author:
Date:
2014-03-20
[Abstract] Staphylococcus aureus has a quorum sensing system to regulate the expression of various virulence factors, which is exerted by the agr locus that encodes agrBDCA and a regulatory RNA called RNAIII. AgrB, AgrD, and AgrC proteins are involved in producing and recognizing extracellular quorum sensing molecules and transduce the signal by altering the phosphorylation status of AgrA, which is a positive transcription factor, to regulate cytolysin genes as well as the RNAIII gene. RNAIII regulates the expression of various virulence genes. Expression of ...
[摘要] 金黄色葡萄球菌具有调节各种毒力因子的表达的群体感应系统，其通过编码agrBDCA的agr基因座和调节agrBDCA的基因座发挥作用。 RNA称为RNAIII。 AgrB，AgrD和AgrC蛋白参与产生和识别胞外群体感应分子并通过改变作为阳性转录因子的AgrA的磷酸化状态来转导信号，以调节细胞溶素基因以及RNAIII/em>基因。 调节各种毒力基因的表达。已经在转录水平上深入研究了 agr 基因座的表达，但是翻译表达的研究是有限的，因为用于检测特异性蛋白的免疫球蛋白G对于高度反应。 aureus 蛋白A.在这里，我们报告检测AgrA的方法，所述AgrA是由 agr 调节系统编码的转录因子。虽然这是用于Western印迹的特定方案。 aureus AgrA蛋白，它也可以用于其他的。金黄色葡萄球菌通过使用适当的抗体。
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