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Author:
Date:
2012-07-05
[Abstract] Many important regulatory proteins such as transcription factors are regulated through subcellular localization. Protein localization can be examined by fusing a GFP tag. However, GFP is relatively big in size, and potentially may affect correct protein localization. Several small tags have been developed, such as myc, HA or Flag. By using immunostain and fluorescence microscopy as described in this protocol, one can easily probe the regulation of a selected yeast protein with the application of the aforementioned small tags.
[摘要] 很多重要调控蛋白如转录因子通过亚细胞定位调控。蛋白定位可以通过融和一个GFP标签来检测。尽管如此，GFP是相当的大的，可能作用于正确的蛋白定位。若干个小标签蛋白已经发展起来，如myc,HA或是Flag。通过使用以下描述的免疫染色和荧光显微镜方法，研究者们可以很容易的检测带有这些小标签酵母蛋白的调控。
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