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Author:
Date:
2014-06-05
[Abstract] Enteric pathogenic bacteria such as Vibrio cholerae and enteropathogenic Escherichia coli (E. coli) cause life-threatening diarrheal diseases that have afflicted humans for centuries. Understanding the effectors required for intestinal colonization is very important to research on bacteria pathogenesis, and is also important to testing new therapeutics and development of the novel vaccines. Here, we describe the Infant Rabbit Colonization Competition Assay, a variant method of the powerful, nonsurgical animal model reported by Ritchie et al. (2010). In our ...
[摘要] 肠道病原性细菌如霍乱弧菌和肠致病性大肠杆菌（大肠杆菌）引起危及人类数百年的危及生命的腹泻疾病。了解肠道定殖所需的效应器对于细菌发病机理的研究是非常重要的，并且对于测试新的治疗剂和新型疫苗的开发也是重要的。在这里，我们描述了婴儿兔殖民化竞争测定，由Ritchie等人（2010）报道的强大的非手术动物模型的变体方法。在我们的修改测定中，野生型和突变株混合在一起，并接种到2日龄新西兰白兔。每个突变体的竞争指数测量突变体相对于其野生型亲本菌株在胃肠道中的定殖能力。与传统的吮吸小鼠模型相比，霍乱弧菌（霍乱弧菌）诱导的婴儿兔的疾病的临床和组织学体征更类似于人类霍乱。该模型的较大输入细菌量也有助于高通量筛选，例如Tn-Seq技术（Fu等人，2013）。
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