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2D Quant Kit

2D定量试剂盒

公司名称: Cytiva
产品编号: 80-6483-56
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In Gel Detection of Lipase Activity in Crude Plant Extracts (Olea europaea)
Author:
Date:
2015-04-20
[Abstract]  Here, we provide a detailed protocol describing a SDS-PAGE based procedure to assay in gel neutral lipase activity. Total protein extracts are separated by SDS-PAGE and gels are treated with lipase substrate-α-naphthyl palmitate. This long-chain fatty acid ester is hydrolysed by lipases present in the gel. The product resulting from this reaction can be then visualized in the gel as yellow-brownish activity bands. This relatively simple and effective method of lipase assay detection can be used for crude protein extracts from different plant tissues. [摘要]  在这里,我们提供详细的协议描述基于SDS-PAGE的程序来测定凝胶中性脂肪酶活性。 通过SDS-PAGE分离总蛋白提取物,并用脂肪酶底物-α-棕榈酸萘酯处理凝胶。 这种长链脂肪酸酯被存在于凝胶中的脂肪酶水解。 由该反应产生的产物然后可以在凝胶中显现为黄棕色活性带。 这种相对简单和有效的脂肪酶测定法可用于来自不同植物组织的粗蛋白提取物。

Analysis of Moraxella catarrhalis Outer Membrane Protein Profiles
Author:
Date:
2013-11-05
[Abstract]  Phenotypes observed for certain Moraxella catarrhalis wild-type strains or mutants may be caused by a variety of factors including alteration of outer membrane protein composition. Examination of the outer membrane protein profiles may be a valuable tool to identify changes in outer membrane compositions of these strains. Here we describe a method to isolate and analyse M. catarrhalis fractions highly enriched for membrane proteins. [摘要]  对某些粘膜炎莫拉菌的野生型菌株或突变体观察到的表型可能由多种因素引起,包括外膜蛋白组成的改变。 外膜蛋白质谱的检查可以是鉴定这些菌株的外膜组成的变化的有价值的工具。 在这里我们描述一种方法来隔离和分析。 粘膜炎的部分高度富集膜蛋白。

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