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OptimaTM XPN

Optima TM XPN

公司名称: Beckman Coulter
产品编号: Optima TM XPN
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Biotinylation and Purification of Surface-exposed Helicobacter pylori Proteins
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2015-04-20
[Abstract]  Interactions between pathogenic bacteria and host cells are often mediated by proteins found on the surfaces of the bacteria. The Gram-negative bacterium Helicobacter pylori is predicted to produce at least 50 surface-exposed outer membrane proteins, but there has been relatively little progress in experimentally analyzing the cell-surface proteome of this organism. Herein, we describe in detail a protocol that allows biotinylation and purification of surface-exposed H. pylori proteins. A comparative analysis of surface-exposed proteins identified by this ... [摘要]  病原细菌和宿主细胞之间的相互作用通常由在细菌表面上发现的蛋白介导。 革兰氏阴性细菌幽门螺杆菌预计产生至少50个表面暴露的外膜蛋白,但是在实验分析该生物体的细胞表面蛋白质组中相对较少的进展。 在本文中,我们详细描述了允许表面暴露的H的生物素化和纯化的方案。 幽门螺杆菌蛋白。 在最近的出版物(Voss等人,2014)中描述了通过这种基于生物素化的方法和几种其它独立方法鉴定的表面暴露蛋白的比较分析。

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