| Generation of Fusarium graminearum Knockout Mutants by the Split-marker Recombination Approach
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Author:
Date:
2018-08-20
[Abstract] Fusarium graminearum is a destructive phytopathogen and shows an impressive metabolic diversity. Gene deletion is an important and useful approach for gene function study. Here we present a protocol for generating gene deletion mutant by applying “split-marker” deletion strategy (Catlett et al., 2003) with PEG-mediated protoplast transformation (Yuan et al., 2008; Martín, 2015).
[摘要] 禾谷镰刀菌是一种破坏性的植物病原体，具有令人印象深刻的代谢多样性。 基因缺失是基因功能研究的重要且有用的方法。 在这里，我们提出了一个协议，通过应用“分裂标记”删除策略（Catlett et al。，2003）与PEG介导的原生质体转化（Yuan 等。，2008;Martín，2015）。
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| Identification of Natural Hybrids by SSR Markers in Mussaenda
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Author:
Date:
2016-07-05
[Abstract] Detection of natural hybrids is of great significance for plant taxonomy, reproductive biology, and population genetic studies. Compared with methods depending on morphological characters, molecular markers provide reliable and much more accurate results. This protocol describes approaches employing microsatellite (SSR) markers to identify inter-specific hybrids in Mussaenda (Rubiaceae).
[摘要] 自然杂交的检测对植物分类学，生殖生物学和群体遗传学研究具有重要意义。 与依赖于形态特征的方法相比，分子标记提供可靠和更准确的结果。 该协议描述了采用微卫星（SSR）标记来鉴定Mussaenda（茜草科）中的特异性杂交体的方法。
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| A Protocol to Measure the Cytoplasmic Calcium in Arabidopsis Guard Cells
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Author:
Date:
2015-05-05
[Abstract] Cytoplasmic calcium ([Ca2+]cyt) acts as a stimulus-induced second messenger in multiple signal transduction cascades (Allen et al., 1999). In plant cells, a dramatic and readily assayed response to stimulus is the change of stomatal aperture. Changes in [Ca2+]cyt of stomatal guard cells were involved in stomatal movement in response to various stimuli and cellular processes. In general, there are two available ways to measure [Ca2+]cyt in guard cells, i.e., loading of calcium-sensitive fluorescence dyes such ...
[摘要] 细胞质钙（[Ca 2+] + cyt）在多个信号转导级联中用作刺激诱导的第二信使（Allen等人， 1999）。在植物细胞中，对刺激的戏剧性和容易测定的响应是气孔孔径的变化。气孔保卫细胞的[Ca + ] 细胞的变化参与响应于各种刺激和细胞过程的气孔运动。一般来说，有两种可用的方法来测量保护细胞中的钙离子荧光染料的加载量，即 例如fluo-3AM和fura-2或表达遗传编码的钙指示剂例如黄色卡梅伦（Krebs等人，2012）。在该方案中，我们旨在描述在ABA或PA处理时加载fluo-3AM的保卫细胞中记录[Ca 2 cyT 用共聚焦激光扫描显微镜进行荧光成像。
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