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Author:
Date:
2015-09-20
[Abstract] The leaf is the major functional part of the shoot performing the bulk of photosynthetic activity. Its development is relatively plastic allowing the plant to adapt to environmental changes by modifying leaf size and anatomy. Moreover, a leaf is made up of various distinct cell layers, each having specialized functions. To understand functional adaptation and the development of the leaf it is essential to obtain cross sections throughout leaf development and at maturity (Kalve et al., 2014). Here, we describe a protocol for transverse sectioning of Arabidopsis thaliana ...
[摘要] 叶是进行大量光合活性的枝条的主要功能部分。其发育是相对塑性的，允许植物通过改变叶的大小和解剖学适应环境变化。此外，叶片由各自不同的细胞层组成，每个细胞层具有专门的功能。为了理解功能适应和叶的发育，必须在叶发育和成熟期间获得横截面（Kalve等人，2014）。在这里，我们描述了使用树脂嵌入的拟南芥叶片的横切片的方案。这个协议提供了一个可靠的平台，产生高质量的横截面图像，允许研究横跨叶片横向轴线的各种组织层的发展。因为该方法是Beeckman和Viane（1999）和De Smet等人（2004）为拟南芥根尖所开发的方案的改编，所以它可以容易地修改以适应其他器官和物种。
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