Skin Wound Healing Model - Excisional Wounding and Assessment of Lesion Area
|
Author:
Date:
2015-11-20
[Abstract] This protocol focus on the most common surgical mouse model of cutaneous excisional wound healing used to study the cellular and molecular pathways involved in wound repair and regeneration as well as in translational applications such as the evaluation of new therapeutic modalities. This model allows the monitoring of the wound closure and the tissue collection for histological and molecular analyses. Briefly, full skin thickness excisional wounds are created on the dorsum of the mouse as the excision extends through the panniculosus carnosus. Wounds larger and minor diameters are ...
[摘要] 该协议侧重于最常见的皮肤切除伤口愈合的外科小鼠模型,用于研究涉及伤口修复和再生以及翻译应用(例如评价新的治疗方式)的细胞和分子途径。 该模型允许监测伤口闭合和组织收集用于组织学和分子分析。 简而言之,当小鼠的背部延伸穿过肉芽肿时,在小鼠的背部产生完全皮肤厚度的切除伤口。 然后定期测量伤口的较大和较小直径,并基于相对于原始尺寸的伤口面积计算伤口闭合率。
|
|
Estimation of Wound Tissue Neutrophil and Macrophage Accumulation by Measuring Myeloperoxidase (MPO) and N-Acetyl-β-D-glucosaminidase (NAG) Activities
|
Author:
Date:
2015-11-20
[Abstract] The inflammatory response is essential to the reestablishment of cutaneous homeostasis following injury. In this context, leukocytes arrive at the wound site and orchestrate essential events in the wound healing process. Therefore, the quantification of specific subsets of inflammatory cells in the wound tissue is of considerable interest. The current protocol focus on a quantitative index of neutrophils and macrophages accumulation within skin lesions by measuring the specific activity of the marker enzymes Myeloperoxidase (MPO) and N-acetyl-β-D-glucosaminidase (NAG), respectively. MPO is ...
[摘要] 炎症反应对于损伤后皮肤稳态的再建立是必需的。 在这种情况下,白细胞到达伤口部位并协调伤口愈合过程中的重要事件。 因此,对伤口组织中炎性细胞的特定亚群的定量是相当感兴趣的。 通过测量标记酶髓过氧化物酶(MPO)和N-乙酰基-β-D-氨基葡萄糖苷酶(NAG)的比活性,目前的方案集中于皮肤损伤中嗜中性粒细胞和巨噬细胞积累的定量指数。 MPO以高水平存在于嗜中性粒细胞和NAG的嗜苯胺粒细胞中,在激活的巨噬细胞的溶酶体中。 这些方法允许间接估计积累到皮肤中的嗜中性粒细胞和巨噬细胞的丰度。
|
|