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DNAse/RNAse free water

DNAse / RNAse游离水

公司名称: Thermo Fisher Scientific
产品编号: 11538646
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Liquid luminescent DNA-precipitation Assay
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2016-05-20
[Abstract]  Working on transcription factors requires studying interactions between protein and DNA. After identification of putative binding-sequences and motifs, Electrophoretic Mobility Shift Assay (EMSA) experiment is classically used to determine specific interactions of proteins and nucleic acids. This lengthy process is rather heavy-handed because of radioisotopically labeled DNA and autoradiographic visualization that are required for the experiments.

Liquid luminescent DNA precipitation assay provides rapid, reliable and quantitative results concerning protein-DNA interactions. This ...
[摘要]  研究转录因子需要研究蛋白质和DNA之间的相互作用。在推定的结合序列和基序的鉴定之后,电泳迁移率变动分析(EMSA)实验通常用于确定蛋白质和核酸的特异性相互作用。这个漫长的过程相当沉重,因为放射性同位素标记的DNA和实验所需的自动放射成像可视化。
 液体发光DNA沉淀测定提供关于蛋白质-DNA相互作用的快速,可靠和定量的结果。这种蛋白质-DNA结合测定基于与洋地黄毒苷标记的(DIG)DNA和结合至谷胱甘肽Sepharose 4B珠粒的谷胱甘肽S-转移酶(GST)融合的DNA结合蛋白(图1)之间的溶液杂交,而不进行电泳(Toshiharu, et al。,,2008)。地高辛是在植物中发现的类固醇。它越来越多地用作核酸和蛋白质的非放射性检测的标签。



图1.液体化学发光DNA下拉试验的表示。谷胱甘肽转移酶(GST)将与谷胱甘肽Sepharose ...

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