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Author:
Date:
2012-11-05
[Abstract] This is a general protocol for the isolation and maintenance of primary MPM cultures as a tool for the identification of Tumor Initiating Cells and early progenitor-targeting drugs (Cioce et al., 2010). Primary cultures can be propagated efficiently for 8-12 weeks and xenotransplanted in NOD/SCID mice while retaining the histofeatures of the originating tumor (Canino et al., 2012). The protocol is suitable for both MPM solid specimens and pleural effusion. For increased clarity, initially two separate sections addressing the isolation of MPM cells from solid tumors and ...
[摘要] 这是用于分离和维持初级MPM培养物的一般方案，作为鉴定肿瘤启动细胞和早期祖细胞靶向药物的工具（Cioce等人，2010）。 原代培养物可以有效繁殖8-12周，并且在NOD/SCID小鼠中异种移植，同时保留起源肿瘤的组织特征（Canino等人，2012）。 该方案适用于MPM固体标本和胸腔积液。 为了增加清晰度，最初提供了两个分开的部分，其涉及从实体瘤和胸腔积液中分离MPM细胞。
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