{{'Search' | translate}}
 

Dimethyl pimelimidate dihydrochloride

庚二亚胺二盐酸盐

公司名称: Sigma-Aldrich
产品编号: D8388
Bio-protocol()
Company-protocol()
Other protocol()

Immunoprecipitation for Cell Culture
Author:
Date:
2012-02-05
[Abstract]  Immunoprecipitation (IP) is a method to pull down a protein out of solution using an antibody that specifically binds to that particular protein. Immunoprecipitation is a powerful technique to isolate and concentrate a particular protein from a sample containing many thousands of different proteins, to test protein-protein interactions, and to pull multiple members of a complex out of solution by latching onto one member with an antibody. This protocol describes a general immunoprecipitation strategy using cell cultures as starting material. [摘要]  免疫沉淀反应是利用特异性结合特定蛋白的抗体从溶液中捕获蛋白的方法。免疫沉淀是一种能从含有数千种不同蛋白的样品中分离浓缩特殊蛋白的有效地技术,可以用来检测蛋白与蛋白之间的相互作用,并且可以通过一个抗体捕获复合物特定蛋白从而从溶液中捕获该复合物的多种蛋白。

产品评论