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Author:
Date:
2012-12-05
[Abstract] Proliferative capacity and degranulation are important features of antigen-specific CD8+ T cells. By combining tetramer staining with a CFSE staining, we were able to enumerate the total number of antigen-specific T cells, as well as their number of divisions upon antigen-specific stimulation during a week. In addition, we performed restimulation of these cells, to analyze their ability to secrete cytolytic granules, visualized by CD107a staining.
[摘要] 增殖能力和脱颗粒是抗原特异性CD8 + T细胞的重要特征。 通过将四聚体染色与CFSE染色组合，我们能够列举抗原特异性T细胞的总数，以及它们在一周内抗原特异性刺激时的分裂数。 此外，我们进行这些细胞的再刺激，以分析其分泌细胞溶解颗粒的能力，通过CD107a染色可视化。
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