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公司名称: Ikemoto Scientific Technology
产品编号: MVX10
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Ex utero Electroporation into Mouse Embryonic Neocortex
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2013-12-20
[Abstract]  This technique allows highly efficient and reproducible transfer of DNA/RNA into the embryonic neocortex of rodents across multiple ages. Ex utero electroporation compliments the more technically difficult in utero electroporation technique by maximizing the number of embryos for available for a given experiment, as well as increasing the variety of constructs used in each experiment, thereby helping to reduce their overall numbers. Ex utero electroporation followed by short term organotypic slice culture of embryonic brain sections allows immediate access to ... [摘要]  这种技术允许高效和可重复地将DNA / RNA转移到多个年龄的啮齿动物的胚胎新皮质中。通过最大化可用于给定实验的胚胎数量,以及增加每个实验中使用的构建体的多样性,从而有助于减少它们的总体数量,子宫内电穿孔在技术上更难以满足子宫电穿孔技术。胚胎电穿孔之后进行短期器官切片培养,可以立即进入多个切片,选择用于活细胞成像实验的最佳切片,以及在完整干细胞生态位中对各种NSC操作的表征。 (参见“子宫内电穿孔小鼠胚胎脑”(Ge,2012);“胚胎脑切片的器官切片培养”(Calderon de Anda,2013)。此外,子宫内电穿孔新生儿可用于体外原代细胞培养进一步解剖,分离成单个细胞,并根据标准技术在盖玻片或多孔培养皿中铺板:注意,该程序可以在电穿孔后立即进行,在异位基因表达发生之前,或在隔膜培养至仅收集电穿孔细胞的区域。

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