| Labeling of Precursor Granule Cells in the Cerebellum by ex vivo Electroporation
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Author:
Date:
2013-06-20
[Abstract] This protocol will be useful to introduce the genes of interest into the cerebellar granule cells at early stages of development. Since the granule cell precursors are localized in the external granule layer before migration, DNA plasmids can be specifically incorporated into the granule cells by injecting DNA solution into the cerebellar fissures followed by application of electric pulses. This technique can be performed prior to the preparation of either dissociated or organotypic culture, which can be used to study the molecular mechanisms of cell migration, axon elongation and ...
[摘要] 该方案可用于在发育的早期阶段将感兴趣的基因引入小脑颗粒细胞。 由于颗粒细胞前体在迁移前位于外部颗粒层中，因此通过将DNA溶液注射到小脑裂隙中，然后施加电脉冲，可将DNA质粒特异性地掺入颗粒细胞中。 该技术可以在制备解离或器官型培养物之前进行，其可以用于研究在发育期间细胞迁移，轴突伸长和突触发生的分子机制。
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| In utero Electroporation of Mouse Embryo Brains
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Author:
Date:
2012-07-20
[Abstract] This is a non-invasive technique to introduce transgenes into developing brains. In this technique, DNA is injected into the lateral ventricle of the embryonic brains, and is incorporated into the cells through electroporation. Embryos then continue their development in normal conditions in vivo. The effects of genes of interest can be evaluated at certain time points after in utero electroporation. This technique allows acute knockdown or over expression of genes of interest. Compensatory effects from other genes are less likely to happen; it also circumvents possible ...
[摘要] 这是一种无侵入性的将基因转入发育的大脑的转基因技术。在这个方法中，DNA被注射入胚胎脑的侧脑室，通过电穿孔的方法被吸收到细胞中。然后，胚胎细胞在体内正常条件继续发育。可以在固定的时间点检测转入的目的基因的在子宫电穿孔中的影响。这种基因允许精确的下调或过表达目的基因。来自于其他基因的补偿性作用很少发生；同时也能避免可能的慢性的有害影响。
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