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Author:
Date:
2014-04-05
[Abstract] We describe fast and reproducible sensitivity assays to quantify the response of Arabidopsis seedlings of different genotypes to a wide range of DNA damaging agents. We apply (1) γ-irradiation, which produces DNA breaks, (2) bleocin, a radiomimetic drug, (3) mitomycin C, a DNA intrastrand cross-linker, (4) hydroxyurea, an inhibitor of DNA synthesis and (5) UV-C, which causes mainly photoproducts. The “true leaf assay” and the “UV resistance assay” are based on easily determined phenotypes as readouts. Using a set of diverse damaging agents combined with different readouts allows ...
[摘要] 我们描述快速和可重复的灵敏度测定以量化不同基因型的拟南芥幼苗对广泛的DNA损伤剂的反应。 我们应用（1）γ辐射，其产生DNA断裂，（2）bleocin，放射性模拟药物，（3）丝裂霉素C，DNA intrastrand交联剂，（4）羟基脲，DNA合成抑制剂， UV-C，其主要产生光产物。 "真叶试验"和"抗UV试验"基于容易确定的表型作为读数。 使用一组不同的损伤剂结合不同的读数允许相对于参考线（例如野生型）建立相对灵敏度/电阻，确定最有效类型的诱导损伤和潜在的修复途径受影响。
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