| In vitro and in vivo Limiting Dilution Assay for Colorectal Cancer
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Author:
Date:
2015-11-20
[Abstract] The in vitro limiting dilution assay is used to determine the colorectal cancer initiating cell (CC-IC) frequency of a CC-IC enriched suspension culture, grown in growth factor enriched serum free media. The in vivo limiting dilution assay is used to determine the colorectal cancer initiating cell frequency of a primary colorectal cancer sample or an established suspension cell line using immunocompromised murine xenograft models. In vitro and in vivo limiting dilution assays (LDAs) can be used to determine the effect of a specific treatment or genetic ...
[摘要] 体外极限稀释测定法用于测定在富含生长因子的无血清培养基中生长的CC-IC富集悬浮培养物的结肠直肠癌起始细胞（CC-IC）频率。 体内限制性稀释测定法用于使用免疫受损的鼠异种移植物模型确定原发性结肠直肠癌样品或建立的悬浮细胞系的结肠直肠癌起始细胞频率。 可以使用体外和体内有限稀释测定法（LDAs）来确定特定处理或遗传击倒策略对CC- IC或结肠直肠癌样品。
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| Isolation and in vitro Activation of Mouse Peyer’s Patch Cells from Small Intestine Tissue
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Author:
Date:
2014-11-05
[Abstract] The lumen of gastrointestinal tract is exposed to several potentially pathogenic microorganisms, thus it is extremely relevant to understand how immunosurveilance can be established. Peyer’s Patches (PPs) are oval or round lymphoid nodules that protrude from the outer wall of the ileum portion of small intestine. PPs contain a high percentage of B and T lymphocytes, macrophages and dendritic cells. Here we summarize a protocol for isolation and culture of mouse PP cells, which can be used to get a better insight into immunopathologies of microbes and to evaluate immune responses elicited by ...
[摘要] 胃肠道的腔暴露于几种潜在的致病微生物，因此，了解如何建立免疫耐受是非常重要的。 派尔氏贴剂（PP）是椭圆形或圆形淋巴结，其从小肠的回肠部分的外壁突出。 PP含有高百分比的B和T淋巴细胞，巨噬细胞和树突细胞。 在这里我们总结一个协议的小鼠PP细胞的分离和培养，可用于更好地了解微生物的免疫病理学和评估由粘膜疫苗引发的免疫反应。
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