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Author:
Date:
2015-05-20
[Abstract] Transient transformation of Artemisia annua does not depend on chromosomal integration of heterologous DNA, and recombinant DNA can be introduced into plant cells via Agrobacterium aided by vacuum. The leaves of 7th and 8th internode from 4-week-old seedlings were chosen as explants, a vacuum system was applied to facilitate agrobacteria into plant cells, the co-cultivation was in the dark at 25 °C for 36-72 h, then GUS or GFP maker genes were used for testing the efficiency of the transformation. The method is used for quick transferring of genes into Artemisia ...
[摘要] 青蒿的瞬时转化不依赖于异源DNA的染色体整合，并且重组DNA可以通过真空辅助土壤杆菌引入植物细胞中。 选择来自4周龄幼苗的第7和第8个节间的叶作为外植体，应用真空系统以将农杆菌促进植物细胞中，共培养在25℃下黑暗中36-72小时，然后 或 GFP 制造商基因用于测试转化的效率。 该方法用于通过瞬时转化将基因快速转移到青蒿（ememansia annua）（ A。annua ）中。
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