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Dual-PAM-100 for measuring chlorophyll fluorescence

双PAM-100测量叶绿素荧光

公司名称: Heinz Walz
产品编号: Dual-PAM-100
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Chlorophyll Fluorescence Measurements in Arabidopsis Wild-type and Photosystem II Mutant Leaves
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2015-07-20
[Abstract]  Chlorophyll fluorescence measurement is a widely used technique to determine photosynthetic performance. Light energy absorbed by a chlorophyll molecule can be dissipated by driving photochemical energy conversion, as heat in non-photochemical quenching processes, or it is re-emitted as fluorescence. The loss of light energy as chlorophyll fluorescence is primarily derived from photosystem II. Photosystem II is a thylakoid-embedded multiprotein complex which provides the high redox potential needed to oxidize water. Within photosystem II photons of light are captured and used to energize ... [摘要]  叶绿素荧光测量是一种广泛使用的技术来确定光合性能。由叶绿素分子吸收的光能可以通过驱动光化学能量转化,作为非光化学淬灭过程中的热量而消散,或者其作为荧光再发射。作为叶绿素荧光的光能的损失主要来自光系统II。光系统II是类囊体嵌入的多蛋白复合物,其提供氧化水所需的高氧化还原电位。在光系统II中,光子被捕获并用于激发电子。激发的电子被馈送到线性电子传输链,并且光系统II用来自水分裂的电子补充丢失的电子。可以使用调制的荧光计设备定量叶绿素荧光产量。在这种装置中,调制的测量光束(以高频率打开和关闭)和仅由测量光激发的荧光的平行检测允许在光合(光化)光的存在下进行叶绿素荧光测量。此外,高强度但短持续时间的光闪烁(饱和脉冲)用于确定在黑暗和光适应叶中的最大荧光产量。在该方案中,给出接受拟南芥野生型叶的典型荧光图的程序。此外,该程序可用于基于各自的荧光图和数值来鉴定影响光系统II的拟南芥突变植物。

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