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Centrifuge

离心机

公司名称: Thermo Fisher Scientific
产品编号: RC5 plus
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Isolation and Growth of Adult Mouse Dorsal Root Ganglia Neurons
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2015-09-20
[Abstract]  Adult dorsal root ganglia neurons are among the few adult neuronal cell types that can be purified and grown relatively easily in dissociated cell culture. Here we describe a procedure for the isolation and growth of dissociated adult mouse DRG neurons using Percoll gradients and a chemically defined medium. These cultures can be used for morphological, biochemical and electrophysiological studies of neuronal growth and function. [摘要]  成年背根神经节神经元是少数成年神经元细胞类型,可以在分离的细胞培养物中相对容易地纯化和生长。 在这里,我们描述了使用Percoll梯度和化学定义的培养基分离和生长分离的成年小鼠DRG神经元的过程。 这些培养物可用于神经元生长和功能的形态学,生物化学和电生理学研究。

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