| cAMP Accumulation Assays Using the AlphaScreen® Kit (PerkinElmer)
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Author:
Date:
2013-07-05
[Abstract] Cyclic adenosine monophosphate (cAMP) is an intracellular signaling messenger derived from the catalytic conversion of ATP, and is a major product of activated Gs protein-coupled receptors. Conversely, formation of cAMP is inhibited by Gi protein-coupled receptors. This protocol has been optimized for the detection of ligand-mediated cAMP accumulation in adherent immortal cell lines expressing Gs-coupled receptors.
[摘要] 环腺苷一磷酸（cAMP）是来源于ATP的催化转化的细胞内信号传导信号，并且是活化的G蛋白偶联受体的主要产物。 相反，cAMP的形成受G 1 i蛋白偶联受体抑制。 该方案已经被优化用于检测表达G s - 偶联受体的贴壁无限增殖细胞系中配体介导的cAMP积累。
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| pERK Detection Assays Using the Surefire AlphaScreen® Kit (TGR Biosciences and PerkinElmer)
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Author:
Date:
2013-07-05
[Abstract] Extracellular signal-regulated kinase 1 and 2 (ERK1/2) are serine/threonine protein kinases that are phosphorylated on Thr202/Tyr204 (ERK1) and Thr185/Tyr187 (ERK2). Phosphorylation of ERK1/2 (pERK1/2) arises from multiple stimuli, resulting in physiological responses that include cell growth, proliferation and differentiation. This protocol has been optimized for the detection of ligand-mediated pERK1/2 in adherent immortal cell lines expressing G protein-coupled receptors (GPCRs).
[摘要] 细胞外信号调节激酶1和2（ERK1/2）是在Thr202/Tyr204（ERK1）和Thr185/Tyr187（ERK2）上被磷酸化的丝氨酸/苏氨酸蛋白激酶。 ERK1/2（pERK1/2）的磷酸化来自多个刺激，导致包括细胞生长，增殖和分化的生理反应。 该协议已经优化用于检测表达G蛋白偶联受体（GPCR）的贴壁永生细胞系中配体介导的pERK1/2。
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